Materials of Construction
Filter Media: 3 μm glass fiber/0.2 μm Supor™ membrane and 3 μm glass fiber/1.2 μm Supor™membrane
Plate Housing: Polyproylene
Lid: Polystyrene
Dimensions
Length: 12.8 cm (5.0 in.)
Width: 8.6 cm (3.4 in.)
Height With Lid: 1.8 cm (0.7 in.) (350 μL only)
Height Without Lid: 350 μL, 1.4 cm (0.6 in.); 1 mL: 3.3 cm (1.3 in.); 2 mL: 4.7 cm (1.9 in.)
Well-Bottom Area
0.25 cm2
Recommended Working Volume
350 μL: < 300 μL
1 mL: < 900 μL
2 mL: < 1.9 mL
Recommended Operating Vacuum
> 25.4 cm Hg (10 in. Hg)
Recommended Centrifugal Force
1,500 x g
Typical Processing Time
PN 8040
Vacuum: 2 seconds
Centrifuge: < 2 minutes
PN 8075, 8175, 8275
Vacuum: 9 seconds
Centrifuge: < 2 minutes
Typical Hold-Up Volume
PN 8040 - Vacuum: 17 μL
PN 8075, 8175, 8275 - Vacuum: 13 μL
Applications
Removal of bacterial debris prior to plasmid purification
Removal of bacterial and cellular debris prior to protein purification
Clearance of gross particulates